Introduction

Next Generation Sequencing

  • DNA Sequencing process

    • Process of determining the order of nucleotides in a genome (genome codes the complete set of instructions for cellular functions).

  • Next Generation Sequencing (NGS)

    • Increasing the throughput by massively parallelising the sequencing process

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Sequencing throughput and sequencing cost

  • Today, we are no longer limited by the availability of sequencing data due to the rapidly decreasing cost

Sequencing cost over the years

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Sequencing throughput over the years

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Main steps of DNA sequencing

graph TD; 1(Extract DNA) --> 2(Prepare DNA for the sequencing machine) 2 --> 3(Sequencing processes) 3 --> 4(Raw sequence data) classDef highlight fill:#99ccff; classDef white fill:#ffffff; class 1,2,3,4 highlight;

Note

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